Skip to main content

Table 3 Summary of the experimental conditions of S. suis co-infection with bacterial pathogens using in vitro models

From: Review of the speculative role of co-infections in Streptococcus suis-associated diseases in pigs

Pathogen studied Type of cells or growth medium Co-infection bacterial strain(s) S. suis serotype 2 strain Co-infection dose Goal of the study Conclusions Refs.
Glaesserella parasuis Neonatal tracheal porcine cell line (NTPr) cells G. parasuis serotype 6 (low virulence) and serotype 5 (high virulence) ST1 (high virulence) and ST25 (intermediate virulence) Adhesion/invasion assays: 5 MOI (both bacterial species)
Gene expression:
10 MOI (both bacterial species)
Adhesion and invasion; expression of inflammatory genes (qRT-PCR) No effect [54]
  Pulmonary alveolar macrophages G. parasuis serotype 6 (low virulence) and serotype 5 (high virulence) ST1 (high virulence) and ST25 (intermediate virulence) Adhesion/invasion assays: 5 MOI (both bacterial species)
Gene expression:
10 MOI (both bacterial species)
*Phagocytosis: 100 MOI (both bacterial species)
Expression of surface markers: 500 MOI/ 50 MOI for G. parasuis/S. suis
Adhesion/invasion, phagocytosis, inflammatory gene expression, and expression of surface markers No effect [54]
Actinobacillus pleuropneumoniae (App) Medium for bacterial culture (no cells) App (no further details on the strain) No details App and S. suis mixed in 1:1 ratio Biofilm formation, antibiotic resistance, and expression of genes for virulent factors Biofilm formation (App), antibiotic resistance and genes coding for different (putative) virulence factors for both pathogens [136]
Bordetella bronchiseptica Porcine precision-cut lung slice Field strain of B. bronchiseptica ST1 (high virulence) 104 and 104 CFU/well for B. bronchiseptica and S. suis, respectively Effect of a pre-infection with B. bronchiseptica on adhesion, colonization and toxicity of S. suis Bacterial adherence, colonization and cell toxicity [137]
  1. *Cells were infected with S. suis first and then with G. parasuis; ST: sequence type.
\