Table 1 Bacteria and plasmids used in this study
Strain/plasmids | Characteristics | References |
|---|---|---|
Strain | ||
CMCC(B)50336 | Salmonella enterica serovar Enteritidis wild-type | NICPBP, China |
â–³ryhB-1mutant | ryhB-1 deficient mutant | This study |
â–³ryhB-2 mutant | ryhB-2 deficient mutant | This study |
â–³ryhB-1/â–³ryhB-2 mutant â–³ryhB-1/pryhB-1 mutant â–³ryhB-2/pryhB-2 mutant â–³ryhB-1/â–³ryhB-2/pryhB-1/pryhB-2 mutant | ryhB-1 and ryhB-2 deficient mutant â–³ryhB-1 carrying pBR-ryhB-1 (Ampr) â–³ryhB-2 carrying pACYC-ryhB-2 (Cmr) â–³ryhB-1/â–³ryhB-2 carrying pBR-ryhB-1 and pACYC-ryhB-2 | This study This study This study This study |
Plasmids | ||
pKD3 | Cmr; Cm cassette template | [34] |
pKD46 | Ampr; λ-red recombinase expression | [34] |
pCP20 | Ampr, Cmr; Flp recombinase expression | [34] |
pGEM-T easy | Ampr; cloning vector | Takara |
pMD19 T-simple pBR-ryhB-1 pACYC-ryhB-2 | Ampr; cloning vector pBR322 carrying the entire ryhB-1 nucleotide sequence pACYC184 carrying the entire ryhB-2 nucleotide sequence | Takara This study This study |
pJV-300 | Ampr; sRNA cloning vector | [40] |
pXG-0 | Cmr; negative control vector without GFP | [40] |
pXG-10SF | Cmr; target gene cloning vector with GFP | [40] |