Effects of RyhB paralogs on the adhesion and invasion of S. Enteritidis. A Adherence to and invasion into Caco-2 cells by SE50336 WT, △ryhB-1 mutant, △ryhB-2 mutant and △ryhB-1/△ryhB-2 mutant. B Adhesion and invasion under SIE in a MEAT model. The black and grey bars represent the numbers of WT, ryhB-1 mutant, ryhB-2 mutant, ryhB-1/ryhB-2 mutant at 0 h (inoculation) and 3 h, respectively. The Y-axis on the left indicates bacterial CFUs, while the Y-axis on the right depicts the ratio of bacteria number at 3 h to the number at 0 h for each strain. Bacterial numbers are represented by bars, while the ratios are shown in a scatter plot. The comparison of invasion ratios between mutants and WT was analyzed statistically using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001.