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Figure 2 | Veterinary Research

Figure 2

From: Positive regulation of Type III secretion effectors and virulence by RyhB paralogs in Salmonella enterica serovar Enteritidis

Figure 2

Growth curves of S. Enteritidis 50336 WT, ryhB-1 mutant, ryhB-2 mutant, and ryhB-1/ryhB-2 mutant in different culture conditions. The number of colony-forming unit per millilitre (CFU/mL) of triplicate cultures were determined at a 2 h interval. Data are the means of three independent experiments. Statistical analysis was performed using unpaired Student’s t-test. Culture conditions of SE50336 WT and all mutants are as follows: A LB broth aerobically with shaking at 160 rpm at 37 °C; B iron-limited LB broth aerobically at 37 °C; C LB broth anaerobically with shaking at 160 rpm at 37 °C; D iron-limited LB broth anaerobically at 37 °C; and E simulated intestinal environment anaerobically at 37 °C.

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