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Figure 9 | Veterinary Research

Figure 9

From: A broadly neutralizing monoclonal antibody induces broad protection against heterogeneous PRRSV strains in piglets

Figure 9

mAb-PN9cx3 administration inhibits PRRSV replication in hilar lymph nodes. A Hilar lymph nodes from the JXA1/mAb-PN9cx3 JXA1/mAb-2G8, JXA1 and MOCK groups were collected at necropsy and embedded in OCT compound in cryomoulds for cryostat sectioning into 5-μm-thick slices. The slices were mounted onto gelatine-coated slides, fixed with ice-cold acetone, and then air-dried. PRRSV antigen was detected using anti-PRRSV nucleocapsid (N) monoclonal antibody (mAb-6D10) and visualized using FITC-labelled goat anti-mouse secondary antibodies. B Hilar lymph nodes from the HNhx/mAb-PN9cx3 HNhx/mAb-2G8 and HNhx groups were collected at necropsy for cryostat sectioning followed by the detection of PRRSV antigen using anti-PRRSV nucleocapsid (N) monoclonal antibody (mAb-6D10). The results were visualized using FITC-labelled goat anti-mouse secondary antibodies.

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Veterinary Research

ISSN: 1297-9716