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Figure 2 | Veterinary Research

Figure 2

From: The lipopolysaccharide outer core transferase genes pcgD and hptE contribute differently to the virulence of Pasteurella multocida in ducks

Figure 2

Schematic strategy used for deletion of the target pcgD (A) or hptE (B) gene in P. multocida. The target gene was replaced with the kanR gene via homologous recombination. The primer pairs DpcgD-1F/1R, DpcgD-2F/2R, and kanR-pcgD-F/R were designed for the construction of PMZ1 (ΔpcgD) and DhptE-1F/1R, DhptE-2F/2R, and kanR-hptE-F/R for the construction of PMZ3 (ΔhptE). The primer pairs P1-F/R, P2-F/R, and P3-F/R were designed for the characterization of PMZ1 (ΔpcgD) and P4-F/P1-R, P2-F/P5-R, and P6-F/R for the characterization of PMZ3 (ΔhptE).

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Veterinary Research

ISSN: 1297-9716