Table 3 Chicken intestinal organoids
Segment | Application | Segment | Crypt isolation | Growth medium | Reference |
|---|---|---|---|---|---|
Caecum | Model development | Caecum | Collagenase, 37 °C, 1.5 h | 50% (DMEM + 10% FBS), 50% L-WRN CM, 10 µM SB431542, 10 µM Y27632 | [14] |
Jejunum | Model development | Jejunum | 2 mM EDTA, 4 °C, 3 × 30 min | DMEM/F12, 10 mM HEPES, 2 mM glutaMAX, 50 ng/mL EGF, 100 ng/mL Noggin, 500 ng/mL R-spondin1 | [40] |
Small intestine | Chemical treatments | Small intestine | Mechanical | DMEM/F12, 10% FBS, insulin-transferrin-selenium, polyamine, bovine pituitary extract | [61] |
Embryonic Small intestine | Model development, TLR agonist and Lactobacillus acidophilus treatment | Embryonic Small intestine | 2.5 mM EGTA, 0.5% glucose, 4 °C, 15 min + 45 min twice | DMEM/F12, Glutamax, insulin-transferrin-selenium premix, 25 ng/mL EGF, 25 ng/mL rh Noggin,250 ng/mL rh R-spondin1, 5 µg/mL Prostaglandin E2 |