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Table 3 Chicken intestinal organoids

From: Intestinal organoids in farm animals

Segment Application Segment Crypt isolation Growth medium Reference
Caecum Model development Caecum Collagenase, 37 °C, 1.5 h 50% (DMEM + 10% FBS), 50% L-WRN CM, 10 µM SB431542, 10 µM Y27632 [14]
Jejunum Model development Jejunum 2 mM EDTA, 4 °C, 3 × 30 min DMEM/F12, 10 mM HEPES, 2 mM glutaMAX, 50 ng/mL EGF, 100 ng/mL Noggin, 500 ng/mL R-spondin1 [40]
Small intestine Chemical treatments Small intestine Mechanical DMEM/F12, 10% FBS, insulin-transferrin-selenium, polyamine, bovine pituitary extract [61]
Embryonic Small intestine Model development, TLR agonist and Lactobacillus acidophilus treatment Embryonic Small intestine 2.5 mM EGTA, 0.5% glucose, 4 °C, 15 min + 45 min twice DMEM/F12, Glutamax, insulin-transferrin-selenium premix, 25 ng/mL EGF, 25 ng/mL rh Noggin,250 ng/mL rh R-spondin1, 5 µg/mL Prostaglandin E2 [15, 31, 41, 42, 62]
  1. CM: conditioned medium, L-WRN: L cell line engineered to secrete Wnt3a, R-spondin 3 and Noggin, rh: recombinant human, FBS: fetal bovine serum, EGF: epidermal growth factor. Antibiotics and antifungals added in the growth medium are not presented.