Effect of TP25 and TP26 on the morphology of HT-29 cell lines. HT-29 cells were treated with 50 μM, 300 μM or 500 μM of TP25 and TP26 for 24 h at 37 °C, then the cells were observed directly (A) or observed after dying with trypan blue (B) under the inverted phase contrast microscope. P86P15 was used as a control. C cytotoxicity of TP25 and TP26 on HT-29 cell lines evaluated by MTT assay. HT-29 cells were treated with different concentrations of TP25, TG 26, or P86P15 for 24 h at 37 °C. Then, the relative inhibition rate of HT-29 cells was determined by MTT assay. Values are shown as mean ± SEM (N = 3). N represents the number of times the experiment was performed. *P < 0.05; **P < 0.01.