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Table 1 List of primers used for sgRNA cloning

From: A novel fiber-2-edited live attenuated vaccine candidate against the highly pathogenic serotype 4 fowl adenovirus

  Sequences of primers (5′–3′)
sgRNA1 F: CACCGGGTTTATCCTTTCGATTACG
R: AAACCGTAATCGAAAGGATAAACCC
sgRNA2 F: CACCGCGTGCTCTACAGCTGTCCAG
R: AAACCTGGACAGCTGTAGAGCACGC