Figure 2

The recombinant virus FA4-EGFP replicated slower than FAdV-4 in vitro. A LMH cells were infected with FA4-EGFP and FAdV-4 at the same dose, respectively, the viral supernatant collected from the infected LMH cells at the indicated time points were then titrated by TCID₅₀. This experiment was done in triplicate and repeated twice. B LMH cells infected with FA4-EGFP and FAdV-4 were harvested at different time points, the lysates of these infected LMH cells were then tested by Western blot with monoclonal antibody 1C9 specific to Fiber-2.