Figure 2

Time kill curves for TYL vs. two isolates of S. delphini (A, B) in MHB over 24 h. The Y-axis presents the bacterial concentration starting from LLOQ as 200 CFU/mL. The X-axis is the time post-inoculation. The error bar at each time point presents the SD for duplicates. Different colors depict the various TYL concentrations with two-fold increase at each step corresponding to 0.25, 0.5, 1, 2, 4 and 8 × MIC. The MIC for both isolates was 0.25 mg/L. The concentrations higher than 2 × MIC resulted in bacterial kill in both isolates. In plot (A), the concentrations of 0.5 and 4 × MIC are masked by 0.25 and 2 × MIC, respectively. In plot (B), the concentrations of 0.25 and 0.5 × MIC are masked by the control (0 × MIC).