Figure 7

Overexpression of PoCD9.1 and its effect on antibacterial infection in FG cells. Overexpression of PoCD9.1. FG cells were administered pCNPoCD9.1, pCN3, and PBS. At 24 h post-transfection, RNA was extracted from the FGs and used for RT-qPCR with primers specific to PoCD9.1. Data are shown as the mean gene expression relative to the expression of the endogenous control β-actin (A). After transfection, the FGs were infected with E. piscicida for 6 h, and the amounts of bacteria were determined (B). Values are shown as the mean ± SEM (N = 3). N, the number of times the experiment was performed. *, P < 0.05, **, P < 0.01.