Primers | Sequences | Purpose |
---|---|---|
Del UL28 kana F | CACACAAATCTTTATATTTTCTCACACGTCGGTTGTGCGGGATTGAAGCAGGATGACGACGATAAGTAGGG | Primers with the UL28 homologous sequences for amplification of the KanR cassette gene |
Del UL28 kana R | ATAAATTGTGTGAGATAAAATGCAGGGACACAAGAATATTGGACGGGTCAGCTTCAATCCCGCACAACCGACGTGTGAGAAAATATAAAGATTTGTGTGCAACCAATTAACCAATTCTGATTAG | |
UL28 BamHI F | GATCGGATCCCAAATGGAGTTGGAGGAC | Primers for PCR identification of the UL28 gene in Figure 3A |
UL28 ApaI R | GATCGGGCCCGTTCTAGACGGATCACGG | |
UL28 XHOI KANA.3 | GATCCTCGAGCAACCAATTAACCAATTCTGATTAG | Primers for construction of the UL28 revertant mutant |
UL28 XHOI KANA.5 | CTCGAGGCATTGGTATCCAAAGAACGTCTTTTCGATCTGAGAACCTCAATAGGATGACGACGATAAGTAGGG | |
Meq Star US | CCGCACACTGATTCCTAG | Primers for PCR and RT-PCR amplification of the meq gene |
Meq End US | CCTTTATGTTGATCTTCCCG | |
Pet30a-UL28 BamHI F | GATCGGATCCTTGGGAATGTCTCATAACCG | Primers for PCR identification of the UL28 gene in Figure 5A |
Pet30a-UL28 NotI R | GATCGCGGCCGCAGATGGGGGCGTGGCTG | |
UL28 F1 | TTTCGATCTGAGAACCTCA | Primers for RT-PCR identification of the UL28 gene in Figure 5B |
UL28 R1 | ATAATGCTGTATTCGACGG | |
Del UL33 kana F | TTCTCCAGAAGATCGGATAAAAGCTGCCAACTCTGTAGGGCGGTTGGCCAGGATGACGACGATAAGTAGGG | Primers with the UL33 homologous sequences for amplification of the KanR cassette gene |
Del UL33 kana R | AACAGTCCTAACAGTCTTTCAAAATTTGATGTAAATCGAGGAACCTATTAGGCCAACCGCCCTACAGAGTTGGCAGCTTTTATCCGATCTTCTGGAGAACAACCAATTAACCAATTCTGATTAG | |
UL33 HindIII F1 | GATCAAGCTTATGCCACATAGGGTTC | Primers for PCR identification of the UL33 gene in Figure 3B |
UL33 BamHI R1 | GATCGGATCCGTTCGGAATGACTTCCATC | |
UL33 PstI Kana.5 | GATCCTGCAGTGAGTAACAATAGCTCCTCAAATTCGTATGGAAGCACCGAGCACGTCTGAGGATGACGACGATAAGTAGGG | Primers for construction of the UL33 revertant mutant |
UL33 PstI Kana.3 | GATCCTGCAGCAACCAATTAACCAATTCTGATTAG |