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Table 1 The primers used in the experiments

From: UL28 and UL33 homologs of Marek’s disease virus terminase complex involved in the regulation of cleavage and packaging of viral DNA are indispensable for replication in cultured cells

Primers

Sequences

Purpose

Del UL28 kana F

CACACAAATCTTTATATTTTCTCACACGTCGGTTGTGCGGGATTGAAGCAGGATGACGACGATAAGTAGGG

Primers with the UL28 homologous sequences for amplification of the KanR cassette gene

Del UL28 kana R

ATAAATTGTGTGAGATAAAATGCAGGGACACAAGAATATTGGACGGGTCAGCTTCAATCCCGCACAACCGACGTGTGAGAAAATATAAAGATTTGTGTGCAACCAATTAACCAATTCTGATTAG

UL28 BamHI F

GATCGGATCCCAAATGGAGTTGGAGGAC

Primers for PCR identification of the UL28 gene in Figure 3A

UL28 ApaI R

GATCGGGCCCGTTCTAGACGGATCACGG

UL28 XHOI KANA.3

GATCCTCGAGCAACCAATTAACCAATTCTGATTAG

Primers for construction of the UL28 revertant mutant

UL28 XHOI KANA.5

CTCGAGGCATTGGTATCCAAAGAACGTCTTTTCGATCTGAGAACCTCAATAGGATGACGACGATAAGTAGGG

Meq Star US

CCGCACACTGATTCCTAG

Primers for PCR and RT-PCR amplification of the meq gene

Meq End US

CCTTTATGTTGATCTTCCCG

Pet30a-UL28 BamHI F

GATCGGATCCTTGGGAATGTCTCATAACCG

Primers for PCR identification of the UL28 gene in Figure 5A

Pet30a-UL28 NotI R

GATCGCGGCCGCAGATGGGGGCGTGGCTG

UL28 F1

TTTCGATCTGAGAACCTCA

Primers for RT-PCR identification of the UL28 gene in Figure 5B

UL28 R1

ATAATGCTGTATTCGACGG

Del UL33 kana F

TTCTCCAGAAGATCGGATAAAAGCTGCCAACTCTGTAGGGCGGTTGGCCAGGATGACGACGATAAGTAGGG

Primers with the UL33 homologous sequences for amplification of the KanR cassette gene

Del UL33 kana R

AACAGTCCTAACAGTCTTTCAAAATTTGATGTAAATCGAGGAACCTATTAGGCCAACCGCCCTACAGAGTTGGCAGCTTTTATCCGATCTTCTGGAGAACAACCAATTAACCAATTCTGATTAG

UL33 HindIII F1

GATCAAGCTTATGCCACATAGGGTTC

Primers for PCR identification of the UL33 gene in Figure 3B

UL33 BamHI R1

GATCGGATCCGTTCGGAATGACTTCCATC

UL33 PstI Kana.5

GATCCTGCAGTGAGTAACAATAGCTCCTCAAATTCGTATGGAAGCACCGAGCACGTCTGAGGATGACGACGATAAGTAGGG

Primers for construction of the UL33 revertant mutant

UL33 PstI Kana.3

GATCCTGCAGCAACCAATTAACCAATTCTGATTAG