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Figure 5 | Veterinary Research

Figure 5

From: UL28 and UL33 homologs of Marek’s disease virus terminase complex involved in the regulation of cleavage and packaging of viral DNA are indispensable for replication in cultured cells

Figure 5

Identification of the MDV ΔUL28 and UL28 gene co-transfected viruses. Cellular DNA and RNA were extracted from the uninfected, recombinant viruses of the parental virus, MDV △UL28, MDV △UL28-Re or MDV △UL28 and UL28 co-transfection produced recombinant viruses infected CEF cells, respectively. A The UL28 and meq gene were PCR amplified using the cellular DNA as template. lane 1and 6 represents uninfected cell; lane 2 and 7 represents parental virus; lane 3 and 8 represents MDV △UL28; lane 4 and 9 represents parental virus; lane 5 and 10 represents MDV △UL28 and UL28 co-transfection; B The UL28 and meq gene were RT-PCR amplified using the cellular RNA as template. lane 1 and 6 represents uninfected cell; lane 2 and 7 represents parental virus; lane 3 and 8 represents MDV △UL28; lane 4 and 9 represents parental virus; lane 5 and 10 represents MDV △UL28 and UL28 co-transfection; C Virus plaque examination of MDV △UL28 and UL28 co-transfection in CEF cells. No plaque was examined and observed in uninfected CEF cells in a (regular light) and b (fluorescent light); MDV specific plaques were examined and observed in the MDV △UL28 and UL28 co-transfected CEF cells in c (regular light) and d (fluorescent light).

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Veterinary Research

ISSN: 1297-9716