Figure 5From: UL28 and UL33 homologs of Marek’s disease virus terminase complex involved in the regulation of cleavage and packaging of viral DNA are indispensable for replication in cultured cellsIdentification of the MDV ΔUL28 and UL28 gene co-transfected viruses. Cellular DNA and RNA were extracted from the uninfected, recombinant viruses of the parental virus, MDV △UL28, MDV △UL28-Re or MDV △UL28 and UL28 co-transfection produced recombinant viruses infected CEF cells, respectively. A The UL28 and meq gene were PCR amplified using the cellular DNA as template. lane 1and 6 represents uninfected cell; lane 2 and 7 represents parental virus; lane 3 and 8 represents MDV △UL28; lane 4 and 9 represents parental virus; lane 5 and 10 represents MDV △UL28 and UL28 co-transfection; B The UL28 and meq gene were RT-PCR amplified using the cellular RNA as template. lane 1 and 6 represents uninfected cell; lane 2 and 7 represents parental virus; lane 3 and 8 represents MDV △UL28; lane 4 and 9 represents parental virus; lane 5 and 10 represents MDV △UL28 and UL28 co-transfection; C Virus plaque examination of MDV △UL28 and UL28 co-transfection in CEF cells. No plaque was examined and observed in uninfected CEF cells in a (regular light) and b (fluorescent light); MDV specific plaques were examined and observed in the MDV △UL28 and UL28 co-transfected CEF cells in c (regular light) and d (fluorescent light).Back to article page