Figure 2From: Identification of genetic determinants of hemolytic activity of Riemerella anatipestifer using random transposon mutagenesisHemolytic activity of the mutants M2 and M18 were restored by plasmid complementation. The WT SX strain, the mutant strains M2 and M18, and the complemented strains M2 (pRES-Riean_0790) and M18 (pRES-Riean_0653) were grown in TSB to the mid-logarithmic phase. The bacterial cell pellets were resuspended in phosphate-buffered saline and 10 μL of the cell suspensions were plated on blood agar. The hemolytic zones generated by these strains were observed. Yb2 (dba− strain) was used as a negative control.Back to article page