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Figure 2 | Veterinary Research

Figure 2

From: Identification of genetic determinants of hemolytic activity of Riemerella anatipestifer using random transposon mutagenesis

Figure 2

Hemolytic activity of the mutants M2 and M18 were restored by plasmid complementation. The WT SX strain, the mutant strains M2 and M18, and the complemented strains M2 (pRES-Riean_0790) and M18 (pRES-Riean_0653) were grown in TSB to the mid-logarithmic phase. The bacterial cell pellets were resuspended in phosphate-buffered saline and 10 μL of the cell suspensions were plated on blood agar. The hemolytic zones generated by these strains were observed. Yb2 (dba strain) was used as a negative control.

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