Figure 5From: Analysis of chicken macrophage functions and gene expressions following infectious bronchitis virus M41 infectionChemokines and inflammatory factors gene expressions. A Chemokine mRNA expressions. Relative mRNA expressions of MIF, CCL4, CXC K60, XCL-1, CXCL12 in IBV-infected, UV-IBV-treated cells and Mock cells were detected by qRT-PCR method. β-actin acted as a reference gene. B Inflammatory factor mRNA expressions. Relative mRNA expressions of IL-1β, IL-6, NF-κB, TNF-α, iNOS, IL-10 and PPAR-γ in IBV-infected, UV-IBV-treated cells and Mock cells were detected by qRT-PCR method. β-actin acted as a reference gene. Data presented as mean ± SD (n = 3). * means the significance of between IBV-infected or UV-IBV-treated cells with Mock cells. * means p < 0.05, ** means p < 0.01.Back to article page