ApaI dendrogram of 24 clusters (similarity ≥ 87% vertical line) of 71 G. anatis isolates, derived from the Dice coefficient and UPGMA using Gelcompar II software. A PFGE cluster was defined as a group of isolates with a similarity of ≥ 87% of their Dice coefficients with position tolerance and optimization set at 1.0% and 2.0%, respectively. The samples are from different sources (Broiler and Battery-cage layer) and different origins (Tehran, Qom, Sari) in Iran. Identical clones among 71 G. anatis isolates from different origins and different production systems were grouped individually. The 21 representative strains for WGS are labelled accordingly (black circle).