Figure 7

PEDV entry requires low pH. A, B Vero cells and IPEC-J2 cells were pre-treated with 30 mM and 50 mM NH4Cl at 37 °C for 1 h and then incubated with GDS01 or GDS09 strains for 1 h. Double-distilled water was used as a negative control. The cells were collected 6 hpi and 9 hpi for qRT-PCR and Western blotting assay, respectively, to test the invasion efficiency of PEDV. C, D The Vero cells and IPEC-J2 cells were pre-treated with 200 nM and 400 nM Baf A1 at 37 °C for 1 h and then incubated with GDS01 or GDS09 strains for 1 h. DMSO was used as a negative control. The cells were collected 6 hpi and 9 hpi for qRT-PCR and Western blotting assay, respectively, to test the invasion efficiency of PEDV. **0.05 < P < 0.01; ***0.01 < P < 0.001; ****P < 0.001.