Figure 6

PEDV utilizes lipid rafts to enter cells. Vero cells (A) and IPEC-J2 cells (B) were incubated or not with PEDV at 37 °C for 1 h and then lysed in TNE buffer containing 1% Triton X-100 and 1% PMSF on ice for 30 min. After mixing with isometric 80% sucrose, the homogenized cell lysates were subjected to ultracentrifugation after being overlaid with 30% and 5% sucrose. After centrifugation, a total of 12 fractions were collected from the top to the bottom of the tubes. The localizations of the lipid raft-associated protein caveolin-1 and PEDV N protein were analyzed by Western blotting after being concentrated with 6% PEG6000.