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Figure 4 | Veterinary Research

Figure 4

From: PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-/lysosome pathway

Figure 4

PEDV entry relies on cholesterol. A, B Vero cells and IPEC-J2 cells were pre-treated with 1.5 mM and 3 mM and 1 mM and 1.5 mM MβCD, respectively, at 37 °C for 1 h and incubated with GDS01 or GDS09 strains for 1 h. Double-distilled water was used as a negative control. The cells were collected at 6 hpi and 9 hpi for qRT-PCR and Western blotting assay, respectively, to test the invasion efficiency of PEDV. C, D The Vero cells and IPEC-J2 cells were pre-treated with 10 μM and 30 μM and 30 μM and 50 μM of nystatin, respectively, at 37 °C for 1 h and incubated with GDS01 or GDS09 strains for 1 h. DMSO was used as a negative control. The cells were collected at 6 hpi and 9 hpi for qRT-PCR and Western blotting assay, respectively, to test the invasion efficiency of PEDV. E The Vero cells and IPEC-J2 cells were pre-treated with different concentrations of MβCD at 37 °C for 1 h, supplemented with 400 μg/mL of soluble cholesterol at 37 °C for 1 h, and infected with PEDV strains for 1 h. The cells were collected at 6 hpi for qRT-PCR assay to test the invasion efficiency of PEDV. *P < 0.05; **0.05 < P < 0.01; ***0.01 < P < 0.001; ****P < 0.001.

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