Figure 2

Dynamin II involved in PEDV entry. A Cells were pre-treated with 30 μM and 50 μM dynasore at 37 °C for 1 h, respectively, and incubated with GDS01 or GDS09 strain for 1 h. DMSO was used as a negative control. The cells were collected at 6 hpi for qRT-PCR assay to test the invasion efficiency of PEDV. B Vero and IPEC-J2 cells were transfected with GFP-Dyn-WT and GFP-Dyn-M, respectively, and infected with PEDV strains at 24 h after transfection. The cells were fixed at 12 hpi and stained for confocal analysis. C, D Vero and IPEC-J2 cells were transfected with siDyn twice and infected with PEDV strains at 24 h after the second transfection. The invasion rates of PEDV into the cells were detected at 6 hpi and 9 hpi for qRT-PCR and Western blotting analysis, respectively. Ctrl means control. Scale bars indicate 25 μm. **0.05 < P < 0.01; ***0.01 < P < 0.001; ****P < 0.001.