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Table 1 Bacterial strains, plasmids, and primers

From: Live vaccine consisting of attenuated Salmonella secreting and delivering Brucella ribosomal protein L7/L12 induces humoral and cellular immune responses and protects mice against virulent Brucella abortus 544 challenge

Strain/PlasmidDescriptionReference
S. Typhimurium
 JOL1800∆lon, ∆cpxR, ∆asd and ∆wpaB mutant of S. TyphimuriumLab stock
 JOL2273JOL1800 containing pJHL65:: L7/L12 and expressing l7/L12Lab stock
 JOL2080JOL1800 carrying the empty vectorLab stock
E. coli
 BL21(DE3)pLysSF, ompT, hsdSB (rB, mB), dcm, gal, λ (DE3), pLysS, CmrProgma, USA
 JOL1921BL21(DE3)pLysS harboring pET28a + :: L7/L12Lab stock
Plasmid
 pET28a(+)IPTG-inducible expression vector; Kanamycin resistantNovagen, USA
 pET28a + :: L7/L12pET28a + derivative containing L7/L12Lab stock
 pJHL65asd+ vector, pBR ori, β-lactamase signal sequence-based periplasmic secretion plasmid, 6xHis, high copy numberLab stock
 pJHL65:: L7/L12pJHL65 harboring L7/L12Lab stock
Primer
 L7/L12 EcoRI F5′-AGAGGAATTCATGGCTGATCTCGCAAAGAT-3′This study
 L7/L12 HindIII R5′-AGAGAAGCTTTTACTTGAGTTCAACCTTGG-3′This study