Skip to main content
Figure 2 | Veterinary Research

Figure 2

From: Live vaccine consisting of attenuated Salmonella secreting and delivering Brucella ribosomal protein L7/L12 induces humoral and cellular immune responses and protects mice against virulent Brucella abortus 544 challenge

Figure 2

Cell mediated immune responses. A Stimulation index. Mice were immunized with PBS, vector-only strain, and Salmonella secreting Brucella L7/L12. Two weeks post-immunization, 5 mice from each group were sacrificed, and splenocytes were aseptically collected and restimulated with immunizing antigen. Cell proliferative response was measured by MTT based assay after 3 days of incubation. ***Indicates significant difference compared to PBS control; ns: non-significant difference. Significance was determined at P < 0.05. Exact probability is demonstrated on graph. B Stimulation index at 3 weeks. The stimulation index was determined at three weeks of immunization against purified L7/L12 antigen and whole Brucella cell lysate. ***Indicates the significant difference against the PBS control, ns: non-significant difference. C Cytokine response. Mice were immunized with PBS, vector-only strain, and Salmonella secreting Brucella L7/L12. Two weeks post-immunization, 5 mice from each group were sacrificed, and splenocytes were aseptically collected and restimulated with immunizing antigen. After 1 day of incubation, interferon-gamma and IL-4 response were evaluated at mRNA level by quantitative RT-PCR. Fold change of expression is demonstrated. ***Indicates significant difference against PBS control group. Level of significance was determined at P < 0.05. Exact probability value is demonstrated on the graph. D Flow cytometry. Mice were immunized with PBS, vector-only strain, and Salmonella secreting Brucella L7/L12. Two weeks post-immunization, 5 mice from each group were sacrificed, and splenocytes were aseptically collected and restimulated with immunizing antigen. After 1 day of incubation, cells were labeled with CD3+. CD4+ and CD8+ specific anti mouse antibodies CD3+: PE, CD4+: PerCP Vio700, CD8+: FITC (Miltenyi Biotec, Gladbach, Germany) and analyzed in Macsquant flow cytometer instrument (Miltenyi Biotec, Gladbach, Germany). Each population was gated and quantified. ***Indicates significant difference against PBS control group.

Back to article page