Figure 4

GST pulldown assay. In vitro GST pulldown assays indicating interactions between TonB and the putative TBDRs (IreA, 0007, 0008, 2235) were performed. Incubation of pGEX-4t-1 (no gene fragment inserted) with a putative protein was used as a negative control. A SDS-PAGE. TBDRs with the His tag (TBDRs-His) are highlighted using black boxes; B Western blot. All His tags are marked with black boxes. M: protein marker (kDa); 1: TonB + 0007; 2: pGEX-4t-1 + 0007; 3: TonB + 0008; 4: pGEX-4t-1 + 0008; 5: TonB + 2235; 6: pGEX-4t-1 + 2235; 7: TonB + IreA; and 8: pGEX-4t-1 + IreA.