DuDDX1 inhibits NDRV virus activity. A Transfected pcDNA3.0-duDDX1-Flag or empty vector in DEF cells for 24 h and then the cells were infected with 10 TCID50/mL NDRV. B SiRNA interference efficiency. DEF cells were transfected with Si-duDDX1-1 as the experimental group or transfected with Si-NC as the control group. The cells were collected at 36 hpt. QRT-PCR was used to detect the expression level of duDDX1 mRNA. C Transfected Si-duDDX1 or Si-NC in DEF cells for 36 h and then the cells were infected with 1 TCID50/mL NDRV. The culture supernatant (A, B are the same) were collected, and detected the virus titer at 24, 36, and 48 hpi by qRT-PCR. A student t test was performed to assess the difference. *P < 0.05; **P < 0.01; ***P < 0.001.