Overexpression of duDDX1 induces gene expression of pattern recognition receptors, pro-inflammatory cytokines, interferons and anti-viral molecules in DEF cells. The experimental group was DEF cells transfected with pcDNA3.0-duDDX1-Flag, and the control group was DEF cells transfected with empty vector. Cells were collected at 24, 36, and 48 hpt, and the mRNA expression levels of pattern recognition receptors (TLR2, TLR3, and TLR4), pro-inflammatory cytokines (IL-6, IL-8, IL-1β, and TNF), interferons (IFN-α, IFN-β, and IFN-γ) and ISGs (PKR, OAS, and Mx) were analyzed by qRT-PCR. The relative expression of gene mRNA were calculated using the 2−ΔΔCT method with GAPDH serving as a normalization gene and mean control values as baseline reference. Data are represented as the mean value ± SE of three experiments. A student t test was performed to assess the difference. *P < 0.05; **P < 0.01; ***P < 0.001.