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Table 2 3D Skin models in domestic animals/rent and companions mammals and birds

From: 3D skin models in domestic animals

Type of models Species Specificities Potential applications References
Skin explants from biopsies cultured ex vivo Rabbit Skin ears epidermis organotypically cultured ex vivo in a diffusion chamber Optimisation of explant culture conditions. Skin keratinization model [134]
Pig Ears skin cultivated ex vivo for dermatological pharmacokinetic purposes Tool for topical application of pharmaceutic products [137]
Pig Skin explants obtained from Gottingen minipig cultured in medium. Dermis thickness shaved down from 20 to 2–3 mm Surrogate for human skin for skin safety studies [141]
Pig Pig ear skin explant seeded onto a gelatin coated polycarbonate transwell insert and cultured ex vivo at ALI Tool to assess UV effects, apoptosis or to evaluate the photoprotective capacity of cosmetic formulations [138]
Pig Dorsal skin punches biopsied cultured ex vivo as explants (with a cornified layer) and maintained at ALI Optimization of animal explants cultures methods. May be a tool to study animals skins diseases [133]
Cow Bovine claw epidermis explants. Hard horn removed and the remaining tissue constituted by a dermis and an epidermis (with a cornified layer) was cultivated in medium at ALI on a metal grid Investigation of epidermal keratinization [135]
Sheep Interdigital skin explants cultivated ex vivo Investigation of anaerobic bacterial infection in the skin [136]
Dog Skin punch biopsies cultured ex vivo 2 weeks immersed in serum-medium Study of dog skin physiology or pharmacological responses [145]
Dog Lateral flank/abdominal skin punches. Cornified layer maintained in ALI during culture Optimization of animal explants cultures methods. May be a tool to study animals skins diseases [133]
Chicken Skin explants cultured on chick chorioallantoic membrane (CAM) Development studies on chicken skin appendages [22]
Chicken Embryonic skin explants, cultivated at ALI on a filter based or semi-solid agar Development studies on chicken skin appendages, Simplified model to study responses underlying skin burning [142]
3-D reconstructed epidermis with primary cells Dog Epidermis equivalent obtained from primary keratinocytes cultured at ALI onto a porcine aDED matrix Characterization of a canine epidermis model. May be a tool for dermatological researches [39]
Horse Epidermal-like architecture obtained from culture of keratinocytes on an inert transwell system Tool for modelling skins diseases, drug testing and maybe equine regenerative medicine [41]
3-D reconstructed FTS with primary keratinocytes cells (modified Bell’s method) Sheep Skin equivalent obtained from culture at ALI of primary ovine keratinocytes onto a dermal (primary ovine fibroblasts) collagen matrix. Used for 3D induction of follicles with the incorporation of the whole derma papillae or cultured papilla cells into the skin equivalent Study of chemical that may facilitate hair follicle development [44]
Sheep Skin equivalent obtained from primary lamb keratinocytes (PLKs) culture at ALI 12 days on a living collagen scaffold (type I rat tail) with 3T3 J2 fibroblasts Model of infection in the skin [43]
Dog Skin equivalent obtained from primary canine keratinocytes cultured at ALI onto a dermal (canine primary fibroblasts)-collagen (type I rat tail) lattice Investigate canine skin biology and pathology [38]
Horse Skin equivalent obtained from the seeding of primary horse keratinocytes onto a type-I collagen dermal scaffold as matrix and cultured 2 weeks at ALI Characterization of equine skin equivalent, may be further a tool for equine regenerative medicine [42]
3-D reconstructed epidermis with keratinocytes derived from progenitor cells Dog Reconstruction of a canine epidermis with a stratum corneum.
Canine progenitor epidermal keratinocytes (CPEK) seeded on a living dermal substitute (primary fibroblasts mixed with collagen) and cultured at ALI
Tool to study stratum corneum canine barrier function [147]
Dog Epidermal equivalent obtained from canine progenitor epidermal keratinocytes (CPEK) cultured at ALI onto a type-I collagen dermis equivalent In vitro tool to study tight junctions in dogs [148]
3-D reconstructed epidermis with keratinocytes derived from iPSC Horse Conditional reprogramming of cells. Culture of keratinocytes derived from equine induced pluripotent stem cells (eiPSCs) at ALI Development of equine skins models using conditional reprogramming. Eventual utility for autologous transplantation [149]
3-D reconstructed epidermis with bulge cells-enriched keratinocytes Dog Use of bulge stem cells keratinocytes from canine hair follicles. Cultured at ALI on a collagen-canine fibroblasts lattice May be a model for dog skin regenerative therapy [150]