Figure  4

Eno interacts with RPSA on the cell surface. A Eno-RPSA interaction shown by Co-IP; B Eno-RPSA interaction shown by BiFC (scale bar, 200 μm); C Eno and RPSA were co-located on the cell surface by immunofluorescence analysis (scale bar, 10 μm); D, E Immunoelectron microscopy analysis of Eno-RPSA interaction in Eno-treated hCMEC/D3. The arrow indicates co-located Eno (labelled with 5-nm gold particles) and RPSA (labelled with 10-nm gold particles) on the cell surface (D) and in the cytoplasm (E) (scale bar, 200 nm).