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Table 1 Primers used for the construction of pET-32a-EmROM2 and pVAX1-EmROM2.

From: Rhomboid protein 2 of Eimeria maxima provided partial protection against infection by homologous species

Plasmids

Primers

pET-32a-EmROM2

Forward: 5′-CGGATCCATGGCGCGGGTTCATACTT-3′

Reverse: 5′-CCAAGCTTTCAGGCGCAACTACGGGGGAG-3′

pVAX1-EmROM2

Forward: 5′-CGCGGATCCATGGCGCGGGTTCATACTT-3′

Reverse: 5′-CCGGAATTCTCAGGCGCAACTACGGGGGAG-3′

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Veterinary Research

ISSN: 1297-9716