Table 1 Primer sequences with their corresponding PCR product size.
Genes | Primers sequences (5′-3′) | Restriction recognition sites | PCR product (base pairs) |
|---|---|---|---|
ChNLRP3 | ChNLRP3/600-F1 CGGGGTACCATGGCAGGAGAAGAAAGCACCAT | Kpn I | 600 bp |
ChNLRP3/600-R1 CCGCTCGAGGTCAAACTGTGTGCAGAGGGTCC | Xho I | ||
ChTLR15 | ChTLR15/CDS-F1 ATAGAATTCATGAGGATCCTTATTGGG | EcoR I | 2607 bp |
ChTLR15/CDS-R1 CCGCTCGAGTCATTCCATCTCAATTACA | Xho I | ||
ChTLR15 | ChTLR15-F GGCTGTGGTATGTGAGAATG | – | 113 bp |
ChTLR15-R ATCGTGCTCGCTGTATGA | – | ||
ChMyD88 | ChMyD88-F CTGGCATCTTCTGAGTAGT | – | 76 bp |
ChMyD88-R TTCCTTATAGTTCTGGCTTCT | – | ||
ChNF-κB | ChNF-κB –F TCTGAACAGCAAGTCATCCATAACG | – | 253 bp |
ChNF-κB –R AAGGAAGTGAGGTTGAGGAGTCG | – | ||
ChNLRP3 | ChNLRP3-F GGTTTACCAGGGGAAATGAGG | – | 252 bp |
ChNLRP3-R TTGTGCTTCCAGATGCCGT | – | ||
ChCaspase-1 | ChCaspase-1-F TAAGCACTTGAGACAGCGGGACG | – | 245 bp |
ChCaspase-1-R GGATGTCCGTGGTCCCATTACTC | – | ||
ChIL-1β | ChIL-1β-F TTCCGCTACACCCGCTCACAGT | – | 242 bp |
ChIL-1β-R CCGCTCATCACACACGACAT | – | ||
ChIL-18 | ChIL-18-F CAGCGTCCAGGTAGAAGATAAG | – | 210 bp |
ChIL-18-R TCCTCAAAGGCCAAGAACAT | – | ||
β-actin | β-actin-F GCCAACAGAGAGAAGATGACAC | – | 139 bp |
β-actin-R GTAACACCATCACCAGAGTCCA | – |