Characterization of prepared polyclonal antisera by Western blot. Proteins extracted from chicken cecal tissues were separated by SDS-PAGE, transferred to nitrocellulose membranes, and then reacted with the prepared rabbit polyclonal antisera against ChNLRP3 and ChTLR15. Membranes were washed and then probed with goat anti-rabbit HRP-conjugated IgG antibody (Sigma, USA). The bands for ChNLRP3 and ChTLR15 proteins were observed. A Lane 1, ChNLRP3 protein (85 kDa). M, protein molecular weight marker (Fermentas). B Lane 1, ChTLR15 protein (130 kDa). M, protein molecular weight marker (Fermentas).