Table 1 Primers used for real-time PCR
Gene | Primer sequence (5′-3′) | Orientation |
|---|---|---|
IFN-α | CTCTTCCTCCAGAAACCTGCAA | Forward |
GAGGAAGAATGGGCTTGTTAGTC | Reverse | |
IFN-β | CCACCACAGCTCTTTCCATGA | Forward |
TGAGGAGTCCCAGGCAACT | Reverse | |
IL-1β | AGAGGGACATGGAGAAGCGA | Forward |
GCCCTCTGGGTATGGCTTT | Reverse | |
TNF-a | GCCCTTCCACCAACGTTTTC | Forward |
TCCCAGGTAGATGGGTTCGT | Reverse | |
IL-6 | CCTCGGCAAAATCTCTGCAA | Forward |
TGAAACTCCACAAGACCGGT | Reverse | |
IL-8 | CCTCATTCCTGTGCTGGTCA | Forward |
TGCAAGTTGAGGCAAGAAGAC | Reverse | |
IL-10 | CGGCCCAGTGAAGAGTTTCT | Forward |
TGCCTTCGGCATTACGTCTT | Reverse | |
TLRl | AGATTTCGTGCCACCCTATG | Forward |
CCTGGGGGATAAACAATGTG | Reverse | |
TLR2 | GAGTCTGCCACAACTCAAAGA | Forward |
CAGAACTGACAACATGGGTAGAA | Reverse | |
TLR3 | GAGCAGGAGTTTGCCTTGTC | Forward |
GGAGGTCATCGGGTATTTGA | Reverse | |
TLR4 | TCATCCAGGAAGGTTTCCAC | Forward |
TGTCCTCCCACTCCAGGTAG | Reverse | |
TLR5 | GGTCCCTGCCTCAGTATCAA | Forward |
TGTTGAGAAACCAGCTGACG | Reverse | |
TLR6 | TCAAGCATTTGGACCTCTCA | Forward |
TTCCAAATCCAGAAGGATGC | Reverse | |
TLR7 | TCTGCCCTGTGATGTCAGTC | Forward |
GCTGGTTTCCATCCAGGTAA | Reverse | |
TLR8 | CTGGGATGCTTGGTTCATCT | Forward |
CATGAGGTTGTCGATGATGG | Reverse | |
TLR9 | AGGGAGACCTCTATCTCCGC | Forward |
AAGTCCAGGGTTTCCAGCTT | Reverse | |
TLRl0 | GCCCAAGGATAGGCGTAAAT | Forward |
CTCGAGACCCTTCATTCAGC | Reverse | |
β-actin | TGCTGTCCCTGTATGCCTCT | Forward |
CTTTGATGTCACGCACGATTT | Reverse |