|
Gene
|
Primer sequence (5′-3′)
|
Orientation
|
|---|
|
IFN-α
|
CTCTTCCTCCAGAAACCTGCAA
|
Forward
|
|
GAGGAAGAATGGGCTTGTTAGTC
|
Reverse
|
|
IFN-β
|
CCACCACAGCTCTTTCCATGA
|
Forward
|
|
TGAGGAGTCCCAGGCAACT
|
Reverse
|
|
IL-1β
|
AGAGGGACATGGAGAAGCGA
|
Forward
|
|
GCCCTCTGGGTATGGCTTT
|
Reverse
|
|
TNF-a
|
GCCCTTCCACCAACGTTTTC
|
Forward
|
|
TCCCAGGTAGATGGGTTCGT
|
Reverse
|
|
IL-6
|
CCTCGGCAAAATCTCTGCAA
|
Forward
|
|
TGAAACTCCACAAGACCGGT
|
Reverse
|
|
IL-8
|
CCTCATTCCTGTGCTGGTCA
|
Forward
|
|
TGCAAGTTGAGGCAAGAAGAC
|
Reverse
|
|
IL-10
|
CGGCCCAGTGAAGAGTTTCT
|
Forward
|
|
TGCCTTCGGCATTACGTCTT
|
Reverse
|
|
TLRl
|
AGATTTCGTGCCACCCTATG
|
Forward
|
|
CCTGGGGGATAAACAATGTG
|
Reverse
|
|
TLR2
|
GAGTCTGCCACAACTCAAAGA
|
Forward
|
|
CAGAACTGACAACATGGGTAGAA
|
Reverse
|
|
TLR3
|
GAGCAGGAGTTTGCCTTGTC
|
Forward
|
|
GGAGGTCATCGGGTATTTGA
|
Reverse
|
|
TLR4
|
TCATCCAGGAAGGTTTCCAC
|
Forward
|
|
TGTCCTCCCACTCCAGGTAG
|
Reverse
|
|
TLR5
|
GGTCCCTGCCTCAGTATCAA
|
Forward
|
|
TGTTGAGAAACCAGCTGACG
|
Reverse
|
|
TLR6
|
TCAAGCATTTGGACCTCTCA
|
Forward
|
|
TTCCAAATCCAGAAGGATGC
|
Reverse
|
|
TLR7
|
TCTGCCCTGTGATGTCAGTC
|
Forward
|
|
GCTGGTTTCCATCCAGGTAA
|
Reverse
|
|
TLR8
|
CTGGGATGCTTGGTTCATCT
|
Forward
|
|
CATGAGGTTGTCGATGATGG
|
Reverse
|
|
TLR9
|
AGGGAGACCTCTATCTCCGC
|
Forward
|
|
AAGTCCAGGGTTTCCAGCTT
|
Reverse
|
|
TLRl0
|
GCCCAAGGATAGGCGTAAAT
|
Forward
|
|
CTCGAGACCCTTCATTCAGC
|
Reverse
|
|
β-actin
|
TGCTGTCCCTGTATGCCTCT
|
Forward
|
|
CTTTGATGTCACGCACGATTT
|
Reverse
|
