The distribution of PEDV in neonatal piglets after intranasal inoculation. A Acute watery diarrhea in neonatal piglets after PEDV intranasal inoculation at 56hpi (white arrowhead). B Pathological changes in the intestine after the autopsy from different groups (Nasal-PBS, Nasal-PEDV). C RNA expression levels of PEDV in different tissues of piglets at 60 hpi after PEDV intranasal inoculation. Data are the mean ± SD. Statistical significance was tested using one-way ANOVA. **P < 0.01. D Protein expression of PEDV in different tissues of diarrheic piglets a determined by western blotting with a mouse mAb against N protein. At least three independent experiments were performed. E, F IHC and IFA of the intestine of intranasally inoculated piglets at 60 h. PEDV antigen was located in the jejunum villus (white arrowhead). The scale bar represents 100 μm. Blue, DAPI; green, PEDV.