Figure6

CD3⁺ T cells acquired PEDV from NECs and migrated to the intestine of neonatal piglets via blood circulation. A Schematic model of PEDV-carrying NECs transmission of the virus to T cells. T cells were co-cultured with NECs by contact. B PEDV-infected NECs were co-cultured with CD3⁺ T cells for 4 h. Viral infection in CD3⁺ T cells was detected by PEDV N protein staining. C FACS analyzed the number of PEDV-loaded CD3⁺T cells derived from PBMC and the jejunum in neonatal piglets at 12hpi after intranasal inoculation. D IF analysis examined CD3⁺ T cells carrying PEDV of jejunum in neonatal piglets through intranasal inoculation. The scale bar represents 50 μm. Blue, DAPI; green, PEDV; Red, CD3.