Figure 1From: PEDV infection in neonatal piglets through the nasal cavity is mediated by subepithelial CD3+ T cellsReplication of PEDV in NECs. A RNA expression levels of PEDV infected NECs at different time points was evaluated by RT-qPCR. B Culture supernatants of PEDV infected NECs were collected at different time points. The viral titer in the supernatant was determined with Vero cells with the results expressed as TCID50/mL. C Protein expression of PEDV in the NECs at different time points by western blotting with a mouse mAb against N protein. *Represents a significant difference relative to the 6 h group (P < 0.05), and **Represents an extremely significant difference relative to the 6 h group (P < 0.01).Back to article page