TUNEL
|
N.A
|
In Situ Cell Death Detection Kit, POD, Roche, Germany
|
N.A
|
N.A
|
Proteinase K in heat incubator
|
cCasp3 (Asp175)
|
mAb
|
SignalStain Apoptosis Cleaved Caspase-3 IHC Detection Kit, Cell Signaling, USA
|
5% NGS
|
1:500
|
pH 6 citrate buffer in microwave
|
PRRSV (SDOW 17)
|
mAb
|
Rural Technologies, Brookings, SD, USA
|
2% BSA
|
1:500
|
Protease XIV in water bath
|
CD163* (2A10/11)
|
mAb
|
INIA, Madrid, Spain
|
2% BSA
|
Neat
|
pH 3.2 citrate buffer microwave
|
Arginase-1
|
pAb
|
ThermoFisher Scientific, Barcelona, Spain
|
2% BSA
|
1:100
|
pH 8.5 citrate buffer microwave
|
cCasp8 (Asp391)
|
mAb
|
Cell Signaling; Danvers, MA, USA
|
2% BSA
|
1:100
|
Proteinase K in heat incubator
|
cCasp9 (Asp330)
|
mAb
|
Cell Signaling; Danvers, MA, USA
|
2% BSA
|
1:50
|
Proteinase K in heat incubator
|