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Figure 2 | Veterinary Research

Figure 2

From: Activation of regulated cell death in the lung of piglets infected with virulent PRRSV-1 Lena strain occurs earlier and mediated by cleaved Caspase-8

Figure 2

PRRSV kinetics of viral load and immunohistochemical expression of PRRSV-N-protein, CD163 and Arg-1 in the lung. Graphs display the kinetics of viral load in the lung for Lena and 3249 strains. Viral load is represented by changes in the cycle threshold (Ct) A and the number of PRRSV-N-protein positive cells/mm2 B in the lung. Scatter dot plot shows individual values from each animal, bars and dashed lines indicate the median for each group and time points. Columns show the median with range of PAMs and septal macrophages (PIMs and interstitial macrophages). Photomicrographs of the middle lung lobe show the expression of PRRSV-N-protein in a representative 3249- C and Lena-infected pig D euthanised at 6 dpi (bar, 50 μm). Insets show in detail PRRSV-N-protein+ expression and viral particles (bar, 20 μm). Photomicrographs of the middle lung lobe show the expression of CD163 in a representative control E and Lena-infected F pig euthanised at 6 dpi (bar, 50 μm). Insets show in detail the immunolabelling of CD163 in the cell membrane and cytoplasm of PAMs (bar, 20 μm). Graph displays the number of CD163+ (primary axis) and Arg-1+ (secondary axis) cells/mm2 in the lung for each group and time point (G). Scatter dot plots show individual values from each animal, bars and dashed lines indicate the median for each group and time point. Columns represent the median with range of PAMs and septal macrophages. Photomicrograph of the middle lung lobe show the expression of Arg-1+ PAMs in a representative Lena-infected pig at 13 dpi H (bar, 50 μm). Inset shows in detail Arg-1 expression in PAMs (bar, 20 μm). Black arrows represent PAMs, and black arrow-heads interstitial macrophages.

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