Skip to main content

Table 4 Details of immunological diagnosis in wild bovids.

From: Diagnosis of tuberculosis in wildlife: a systematic review

Assay test Species N/E nSe + nSp Antigens Se (%) Sp (%) References
Skin test (CFT) Wild bison N ND bPPD 57.6 80.3 [105]
  Wild bison N 2 + 24 bPPD 50 88 [111]
Skin test (SCITT) African buffalo N 51 bPPD, aPPD 86.3 NE [88]
IGRA African buffalo N + E 149 + 344 bPPD, aPPD 92.1 68.3 [81]
African buffalo N 8 bPPD, aPPD 100 NE [7]
PC-HP, PC-EC 75 NE
  African buffalo N 44 ESAT-6, CFP-10, TB-7.7 71 NE [86]
PC-EC 91 NE
PC-HP 95 NE
  African buffalo N 51 + 70 ESAT-6, CFP-10, TB-7.7 80.4 100 [88]
IP-10 assay African buffalo N 44 + 40 PC-HP 93.2 90 [86]
PC-EC 81.8 92.5
ESAT-6, CFP-10, TB-7.7 86.4 92.5
  African buffalo N 32 + 39 ESAT-6, CFP-10, TB-7.7 94 92 [87]
  African buffalo N 51 + 70 ESAT-6, CFP-10, TB-7.7 88.2 100 [88]
MAPIA Wild bison N 12 + 70 M. bovis antigensa 92 97 [111]
FPA Wild bison N ND MPB70 4.4 83.2 [105]
Wild bison N 9 + 57 MPB70 67 34 [111]
Idexx ELISA African buffalo N 8 bPPD 37.5 NE [7]
BovidTB STAT-PAK African buffalo N 100 + 100 ESAT-6, CFP-10, MPB83 33 90 [114]
VetTB STAT-PAK Wild bison N ND ESAT-6, CFP-10, MPB83 12.7 98.4 [105]
RT1 African buffalo N 100 + 100 NM 23 94 [114]
RT2 Wild bison N 12 + 70 ESAT-6, CFP-10, MPB83 67 99 [111]
  1. N/E: natural or experimental infection; nSe: number of TB positive animals used for evaluation of Se; nSp: number of TB negative animals used for evaluation of Sp; Se: sensitivity; Sp: specificity; CFT: caudal fold test; SCITT: single comparative intradermal tuberculin skin test; IGRA: interferon gamma release assay; IP-10: interferon gamma-inducible protein 10; PC-EC: contains ESAT-6- and CFP-10-derived peptides; PC-HP contains ESAT-6- and CFP-10-derived peptides and 4 mycobacterial antigens (including Rv3615); MAPIA: multiantigenimmunoprint assay; FPA: fluorescent polarization assay; ELISA: enzyme linked immunosorbent assay; Idexx ELISA: commercial ELISA (Idexx Laboratories, Inc., Westbrook, ME, USA); Bovid TB STAT-PAK and DPP Vet TB: lateral flow tests (Chembio Diagnostic Systems, Inc., USA); RT1: rapid test (Anigen, Animal Genetics, Inc., South Korea-name of the test not mentioned); RT2: lateral flow rapid test (Chembio Diagnostic Systems, Inc., USA); NE: not estimated; NM: not mentioned; ND: not defined, Se and Sp estimated based on Bayesian analysis (absence of gold standard).
  2. a ESAT-6, CFP-10, MPB59, MPB64, MPB70, MPB83, the16-kDa protein, the 38-kDa protein, two fusion proteins comprising CFP-10/ESAT-6 and the 16 kDa protein/MPB83, and two native antigens, bovine PPD and M. bovis culture filtrate.
\