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Table 3 Details of immunological diagnosis in European badger.

From: Diagnosis of tuberculosis in wildlife: a systematic review

Assay test N/E nSe +nSp Antigen Se (%) Sp (%) References
Skin test N 10 + 37 bPPD, NT, whole killed bovine tubercle bacilli 70 73 [64]
IGRA N 46 + 185 bPPD, aPPD 74.5
80.9a
93.6
93.6a
[80]
   ESAT-6/CFP-10 60.9
60.9 a
94.6
94.6 a
 
N ND bPPD, aPPD 79.9 95 [143]
N 39 + 147 bPPD, aPPD 84.6b 92.5b [96]
7 + 40 57.1c 97.5c
N ND bPPD, aPPD 52 97 [142]
LTA N 8 + 13 bPPD, aPPD 87.5 84.6 [92]
qRT-PCR of cytokines expression N 53 + 194 bPPD, ESAT-6/CFP-10 64.2 93.3 [93]
ELISA N 25 + 28 P22 80d, 76e 85.7d, 85.7e [99]
E 34 + 36 82d,79e 80.56d, 83.3e
Brock test N 8 + 13 MPB83 62.5 100 [92]
N 41 + 33 MPB83 46 82 [95]
N 340 + 817 MPB83 54.7b 93b [96]
39 + 238 53.9c 96.6c
N 51 + 193 MPB83 52.9 90.7 [93]
N 78 + 100 MPB83 47.4 89 [101]
Dachs TB ELISA N 41 + 33 MPB83 61 82 [95]
MAPIA N 78 + 100 M. bovis antigensf
M. bovis antigensg
48.7
59
88
84
[101]
BrockTB STAT-PAK N 340 + 817
39 + 238
ESAT-6, CFP-10, MPB83 49.7b
56.4c
92.5b
96.2c
[96]
N ND ESAT-6, CFP-10, MPB83 50.4 96.9 [143]
N NM ESAT-6, CFP-10, MPB83 59.09 66.67 [139]
N ND ESAT-6, CFP-10, MPB83 58 97 [142]
DPP Vet TB N 38 + 418 ESAT-6, CFP-10, MPB83 50 95 [123]
RT N 454 + 1078 ESAT-6, CFP-10, MPB83 50.7 93.1 [109]
  N 78 + 100 MPB83, TBF 10 52.6 95 [101]
  1. N/E: natural or experimental infection; nSe: number of TB positive animals used for evaluation of Se; nSp: number of TB negative animals used for evaluation of Sp; Se: sensitivity; Sp: specificity; IGRA: interferon gamma release assay; ELISA: enzyme linked immunosorbent assay; LTA: comparative lymphocyte transformation assay; qRT-PCR: quantitative reverse-transcription polymerase chain reaction assay; MAPIA: multiantigenimmunoprint assay; NT: new tuberculin; Brock TB STAT-PAK: lateral flow rapid test (Chembio Diagnostic Systems, Inc., USA); RT: rapid test (name not mentioned).
  2. aSe and Sp based on rabbit monoclonal antiserum (mEIA) instead of polyclonal pair (pEIA) of antibodies in other studies; ND: not defined, Se and Sp are calculated based on Bayesian analysis; NM: not mentioned in the article; NE: not estimated.
  3. bSe or Sp in adults.
  4. cSe or Sp in cubs.
  5. dIndirect ELISA.
  6. eCompetitive ELISA.
  7. fRecombinant antigens only i.e., ESAT-6, CFP-10,MPB59, MPB64, MPB70, MPB83, 16-kDa protein, 38-kDa protein, CFP-10/ESAT-6 and 16 kDa protein/MPB83.
  8. gRecombinant antigens and M. bovis culture filtrate.
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