Diagnosis of tuberculosis in wildlife: a systematic review

Thomas, Jobin; Balseiro, Ana; Gortázar, Christian; Risalde, María A.

doi:10.1186/s13567-020-00881-y

Veterinary Research

Table 1 Details of immunological diagnosis in Cervidae.

From: Diagnosis of tuberculosis in wildlife: a systematic review

Assay test	Species	N/E	n_Se + n_Sp	Antigens	Se (%)	Sp (%)	References
Skin test (SCITT)	Red deer	N + E	60 + 1157	bPPD, aPPD	91.4	98.7	[144]
	Red deer	N	218	bPPD, aPPD	NE	46.9	[126]
	Elk	N	7 + 3	bPPD, aPPD	100	100	[145]
	Reindeer	E	13 + 4	bPPD, aPPD	92	25	[146]
	White tailed deer	N + E	60 + 56	bPPD, aPPD	97	81	[61]
	Fallow deer	N	21	bPPD, aPPD	80.1	NE	[21]
IGRA	White tailed deer	E	91 + 44	bPPD, aPPD	74	98	[83]
	Elk	N	51	bPPD, aPPD	NE	90^a, 78^b	[79]
	Elk			ESAT-6/CFP-10	NE	100^c 96^d	[79]
	Reindeer	N	106	bPPD, aPPD	NE	91^a 83^b
	Reindeer			ESAT-6/CFP-10	NE	94^c, 87.5^d
	White tailed deer	N	95	bPPD	NE	98^a, 92^b
	White tailed deer			ESAT-6/CFP-10	NE	97^c, 95^d
	Red deer	E	15	bPPD	92.8^e, 100^f, 75^g	100	[23]
				P22	92.8^e, 100^f, 87.5^g	100
				ESAT-6/CFP-10	92.3^e, 100^f, 75^g	100
				Rv3615c	40^e, 26.6^f, 37.5^g	100
				Rv3020c	66.6^e, 100^f, 87.5^g	100
LST	Elk	N	66 + 324	bPPD	70	74	[147]
	Elk	N	33 + 450	bPPD	83	64	[104]
	Red deer	N	39 + 16	bPPD	95	44	[68]
				MPB70	72	50	[68]
	Red deer-elk hybrid	E	10 + 15	bPPD	65.7	92.5	[84]
qRT-PCR of cytokines expression	Red deer-elk hybrid	E	10 + 15	bPPD	78.6	97.5	[84]
ELISA	Red deer	N	104 + 56	bPPD MPB70	88 80	52 79	[68]
	Red deer	N	94 + 217	bPPD, MPB70, aPPD	45.7	100	[126]
	Red deer	E	15 + 15	Ethanol Extract of M. bovis	86.7	93.3	[102]
	Red deer	N	221 + 204	bPPD P22	70.1 70.1	91.6 99	[24]
	Elk	N	108 + 48	MPB83	49.1	97.9	[138]
	Fallowdeer	N	73 + 157	bPPD	51	96	[120]
	White tailed deer	N	12 + 329	LAM enriched antigen	66.7^h, 58.3ⁱ	95.1^h 97.3ⁱ	[113]
	Reindeer	E	11 + 4	LAM	100	50	[55]
BTB	Red deer	N	87 + 200	bPPD, aPPD	90.8	98	[126]
FPA	Red deer and elk	N	16	MPB70	81	NE	[91]
	Elk	N	33 + 450	MPB70	40	81	[104]
	Red deer-elk hybrid	E	10	MPB70	33.33	NE	[75]
RT	White tailed deer	N/E	28 + 435	ESAT-6, CFP-10, MPB83	75	98.9	[109]
Cervid TB STAT-PAK	Elk	N	31 + 842	ESAT-6, CFP-10, MPB83	87.1	98.3	[148]
	Elk	N	33 + 450	ESAT-6, CFP-10, MPB83	62	87	[104]
	Elk Fallow deer	N	34 + 141 32 + 107	ESAT-6, CFP-10, MPB83	82 91	93 91	[107]
	Fallow deer	N	21	ESAT-6, CFP-10, MPB83	80.1	NE	[21]
	Red deer-elk hybrid	E	10	ESAT-6, CFP-10, MPB83	72.5	NE	[75]
	White tailed deer	N	22 + 724	ESAT-6, CFP-10, MPB83	54.5	98.1	[113]
	Red deer	N/E	52 + 105	ESAT-6, CFP-10, MPB83	86.5	83.8	[119]
	Mixed Deer sp.	N	7 + 425	ESAT-6, CFP-10, MPB83	85.7^j	94.8^j	[119]
DPP Vet TB	Elk Fallow deer	N	34 + 141 32 + 107	ESAT-6, CFP-10, MPB83	79 91	98 99	[107]
	Fallow deer	N	73 + 157	ESAT-6, CFP-10, MPB83	71	88	[120]
	Red deer	N/E	52 + 105	ESAT-6, CFP-10, MPB83	84.6	91.4	[119]
	White tailed deer	N/E	63 + 903	ESAT-6, CFP-10, MPB83	65.1	97.8	[121]
MAPIA	Elk Fallow deer	N	34 32	M. bovis antigens^k	82 97	NE NE	[107]
	Red deer-elk hybrid	E	10	M. bovis antigens^k	76.7	NE	[75]
	White tailed deer	N	22 + 727	M. bovis antigens^k	68.2	97.1	[113]
	Reindeer	E	11 + 23	M. bovis antigens^k	100	85	[55]
IB	White tailed deer	N	20 + 671 13 + 333	MPB83 Whole cell sonicate	55 46.2	99.3 92.5	[113]
IB	Reindeer	E	11 + 4	Whole cell sonicate	90.9	50	[55]

N/E: natural or experimental infection; n_Se: number of TB positive animals used for evaluation of Se; n_Sp: number of TB negative animals used for evaluation of Sp; Se: sensitivity; Sp: specificity; SCITT: single comparative intradermal tuberculin skin test; LST: lymphocyte stimulation test; qRT-PCR: quantitative reverse-transcription polymerase chain reaction assay; IGRA: interferon gamma release assay; ELISA: enzyme linked immunosorbent assay; BTB: blood tuberculosis test; FPA: fluorescent polarization assay; MAPIA: multiantigenimmunoprint assay; Cervid TB STAT-PAK and DPP Vet TB: lateral flow tests (Chembio Diagnostic Systems, Inc., USA); RT: lateral flow rapid test (name not mentioned-Chembio Diagnostic Systems, Inc., USA); NE: not estimated; IB: immunoblotting
^a Cut off: OD of bPPD–aPPD and bPPD–PBS > 0.1.
^b Cut off: OD of bPPD–aPPD and bPPD–PBS > 0.05.
^c Cut off: OD of ESAT-6:CFP-10–PBS > 0.1.
^d Cut off: OD of ESAT-6:CFP10–PBS > 0.05.
^e Se of experimentally infected animals at 15 days post-infection (dpi).
^f Se of experimentally infected animals at 30 dpi.
^g Se of experimentally infected animals at 60 dpi (cut off for e, f, g: for bPPD and P22, OD of bPPD–aPPD and bPPD–PBS > 0.05, for specific antigens like ESAT-6:CFP10, Rv3615c and Rv3020c, OD of specific antigen–PBS > 0.05).
^h Cut off: OD ≥ 0.25.
ⁱ Cut off: OD ≥ 0.3.
^j Se and Sp were evaluated based on mixed species of deer consisting of fallow deer, roe deer and red deer.
^k ESAT-6, CFP-10, MPB59, MPB64, MPB70, MPB83, 16-kDa protein, 38-kDa protein, CFP-10/ESAT-6 and 16 kDa protein/MPB83, and two native antigens, bovine PPD and M. bovis culture filtrate.

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ISSN: 1297-9716

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