Figure 4

S/P ratios of PEDV IgA antibody in culture supernatants of MNCs isolated from the blood, spleen, MLN, upper jejunum and ileum of PEDV-inoculated pigs, with or without in vitro stimulation. MNCs were isolated from the blood (A), spleen (B), MLN (C), upper jejunum (D) and ileum (E) of PEDV-inoculated pigs. The MNCs were cultured for 4 days to evaluate the responses of specific ASCs. In the other wells, the MNCs were cultured with R848 for 4 days for stimulation of memory B cells. Cell supernatants were collected and PEDV-specific antibodies in the supernatants were detected by an IgA ELISA. *p < 0.05 vs. unstimulated group, by Dunnett's test. ^†p < 0.1, *p < 0.05 and **p < 0.01 (suggestive, significant, and highly significant differences, respectively) for unstimulated vs. stimulated groups, by one-tailed Student's t-test. Black bar: without in vitro stimulation. Open bar: with in vitro stimulation.