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Figure 6 | Veterinary Research

Figure 6

From: N-linked glycosylation at site 158 of the HA protein of H5N6 highly pathogenic avian influenza virus is important for viral biological properties and host immune responses

Figure 6

Transcriptome analysis of differential expression genes (DEG) in RY6- and RY6-160 T-infected A549 cells. A Number of DEG in A549 cells infected with RY6 and RY6-160 T relative to mock-infected A549 cells (q-value <  = 0.05, Fold-change >  = 2). The number of DEG was labeled with color (Red refers to up-regulated, blue refers to down-regulated). B Venn diagram showed the distribution of DEG in A549 cells during infection with RY6 and RY6-160 T. C The most significantly enriched pathways and classic pathways activated by RY6 and RY6-160 T. D Scatter plot of GO enrichment for DEG. The higher the Rich Factor, the higher the degree of enrichment. Q-value represents the p-value after multiple hypothesis test correction. The less the value, the higher degree of enrichment. E Validation of microarray gene expression data by means of RT-qPCR analysis. All values were normalized to Gapdh and are expressed as fold change compared with controls. The values represent means ± SD from three independent experiments. (*p<0.05, **p<0.01, ***p<0.001).

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Veterinary Research

ISSN: 1297-9716