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Figure 4 | Veterinary Research

Figure 4

From: N-linked glycosylation at site 158 of the HA protein of H5N6 highly pathogenic avian influenza virus is important for viral biological properties and host immune responses

Figure 4

Solid-phase receptor-binding assay and virus-cell binding assays of four H5N6 viruses. AD The binding of H5N6 viruses to two different biotinylated glycans was tested in a solid-phase binding assay. The data shown are the means of three replicates; the error bars indicate standard deviations. E A549 cells were seeded and inoculated at an MOI of 5 with four tested viruses. Virus-bound and unbound cells were analyzed by flow cytometry. F Percentages of virus-bound cells as determined by using GraphPad software. Values represent means ± SD from three independent experiments. (*p < 0.05, **p < 0.01).

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