Figure 1From: N-linked glycosylation at site 158 of the HA protein of H5N6 highly pathogenic avian influenza virus is important for viral biological properties and host immune responsesPathogenicity of four H5N6 viruses in mice and replication of RY6 and RY6-160 T in mice. Mean weight of mice infected from 103.0 EID50/50 μL to 107.0 EID50/50 μL of RY6 (A), RY6-160T (B), RHX (E) and RHX-160A (F) (n = 5). Survival rate of mice infected with RY6 (C), RY6-160T (D), RHX (G) and RHX-160A (H); mice were humanly killed when they lost ≥ 25% of their initial body weight. (I and J) Six-week-old SPF BALB/c mice (three/group) were inoculated intranasally with 106 EID50 of RY6 and RY6-160T, and organs were collected on days 3 and 5 post-infection for virus titration in eggs. Values represent means ± SD from three independent experiments. (**p < 0.01, ***p < 0.001).Back to article page