Figure 4From: Biological properties and roles of a Trichinella spiralis inorganic pyrophosphatase in molting and developmental process of intestinal larval stagesqPCR and Western blot analysis of TsPPase transcription and expression at various T. spiralis stages. The transcription levels were calculated with the Ct (2−ΔΔCt) method. *P < 0.05 compared with IIL stage worms (A). The expression level at IIL stage was the highest, the difference had statistical significance. *P < 0.05 compared with other stage worms (B). GAPDH was utilized as an internal control.Back to article page