Table 2 Primers used for cloning PCR
Gene product | Sense primer (5′–3′) | Antisense primer (5′–3′) |
|---|---|---|
PCV3 Cap(1-214aa) | ATGAGACACAGAGCTATATTC | TTAGAGAACGGACTTGTAACGAATC |
PCV3 Cap(35-214aa) | ATGGCTGGCACATACTACACAAAGAAAT | TTAGAGAACGGACTTGTAACGAATC |
PCV3 Cap(1-34aa) | ATGAGACACAGAGCTATATTC | TTA TGTGGGCCTCCTAATGAATA |
NPM1(1-294aa) | ATGGAAGATTCGATGGATAT | TTAAAGAGACTTCCTCCACT |
NPM1(1-117aa) | ATGGAAGATTCGATGGATAT | TACTAAGTGCTGTCCACTAATATGC |
NPM1(118-188aa) | GCTGTAGAGGAAGATGCAGAGT | TTCCGCTTCCTCATCATCAAA |
NPM1(189-294aa) | GAAAAAGCTCCAGTAAAGAAAT | TTAAAGAGACTTCCTCCACT |
NPM1(1-188aa) | ATGGAAGATTCGATGGATAT | TTCCGCTTCCTCATCATCAAA |
NPM1(118-294aa) | GCTGTAGAGGAAGATGCAGAGT | TTAAAGAGACTTCCTCCACT |
NPM1(1-117aa + 189-294aa) | ATGGAAGATTCGATGGATAT | CTTTACTGGAGCTTTTTCTACTAAGTGCTGTCCACTA |
TAGTGGACAGCACTTAGTAGAAAAAGCTCCAGTAAAG | TTAAAGAGACTTCCTCCACT | |
ATGGAAGATTCGATGGATAT | TTAAAGAGACTTCCTCCACT | |
NPM1-S48A | TTATCTTTAAGAACGGTCGCATTAGGGGCT | AGCCCCTAATGCGACCGTTCTTAAAGATAA |
NPM1-S48E | TTATCTTTAAGAACGGTCGAATTAGGGGCTGGCGCAAA | TTTGCGCCAGCCCCTAATTCGACCGTTCTTAAAGATAA |
NPM1-S88A | AACTTTGAAAATGTCTGTACAGCCGACGGTTGCACTTG | CAAGTGCAACCGTCGGCTGTACAGACATTTTCAAAGTT |
NPM1-S88E | AACTTTGAAAATGTCTGTACAGCCGACGGTTGAACTTG | CAAGTTCAACCGTCGGCTGTACAGACATTTTCAAAGTT |
NPM1-T95A | TTGGGGGCTTTGAAATAGCACCACCTGTGGTCTTA | TAAGACCACAGGTGGTGCTATTTCAAAGCCCCCAA |
NPM1-T95D | TTGGGGGCTTTGAAATAGATCCACCTGTGGTCTTA | TAAGACCACAGGTGGATCTATTTCAAAGCCCCCAA |
NPM1-S48T | TTATCTTTAAGAACGGTCACATTAGGGGCT | AGCCCCTAATGTGACCGTTCTTAAAGATAA |
NPM1-S48D | AAAATGAGCACCAGTTATCTTTAAGAACGGTCGATTTA | TAAATCGACCGTTCTTAAAGATAACTGGTGCTCATTTT |
NPM1-S48K | TTATCTTTAAGAACGGTCAAATTAGGGGCTGGCGCAAA | TTTGCGCCAGCCCCTAATTTGACCGTTCTTAAAGATAA |
NPM1-S48R | TTATCTTTAAGAACGGTCAGATTAGGGGCTGGCGCAAA | TTTGCGCCAGCCCCTAATCTGACCGTTCTTAAAGATAA |