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Figure 5 | Veterinary Research

Figure 5

From: The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein

Figure 5

Charge property of serine-48 within NPM1 determines its subcellular localization and interaction with PCV3 Cap. A, B Charge property of serine-48 of NPM1 is responsible for binding to PCV3 Cap. HEK293T cells were cotransfected with NPM1 or NPM1 mutants (-S48E, -S48D, -S48A, -S48T, -S48K, or -S48R), along with Flag-PCV3-Cap and Flag-gst-PCV3-Cap expression vectors, and the cell lysates were subjected to immunoprecipitation or GST pull-down and immunoblotting using the indicated antibodies. C Nucleolin colocalization with NPM1 and various NPM1 mutants in transfected cells. HEK293T cells were cotransfected with GFP-NPM1 or NPM1 mutants (-S48E, -S48D, -S48A, -S48T, -S48K, or -S48R), along with mCherry-NCL for 24 h, and cells were fixed and then subjected to confocal microscopy analysis. Nuclei were stained with 4′, 6′-diamidino-2-phenylindole (DAPI). Scale bar, 10 μm.

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Veterinary Research

ISSN: 1297-9716