Figure 3
From: Specific EtMIC3-binding peptides inhibit Eimeria tenella sporozoites entry into host cells
Binding detection of three target phages with recombinant EtMIC3-bc1 protein and sporozoites protein. ELISA (A, C) and competitive ELISA (B) were applied to assay the binding capability of three phages A, D and W with recombinant EtMIC3-bc1 protein and sporozoites protein. The phages A, D and W diluted from 1.0 × 106 to 1.0 × 1012 pfu were coated in 96-well plate, respectively, and the three phages showed higher binding capability to EtMIC3-bc1 protein than the unrelated phages from G protein of Vesicular stomatitis virus (VSV) (negative control) (p < 0.01) (A). The specific antisera against EtMIC3-bc1 protein significantly inhibited binding of the three phages to EtMIC3-bc1 protein compared with the irrelevant antibody against hexon protein of fowl adenovirus serotype 4 (FADV-4) (negative antibody control) (p < 0.01) (B). The phages A, D and W displayed specific binding with sonicated sporozoites protein. The binding of the three phages to recombinant EtMIC3-bc1 protein was used as positive control, and meanwhile the binding of unrelated phages from G protein of VSV to sporozoites was used as negative control to exclude the possibility of binding between any other phages and sporozoites protein (C). Each sample was tested in triplicate. Values represent mean ± SD. ∗ P < 0.05, ∗  ∗ P < 0.01.