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Figure 2 | Veterinary Research

Figure 2

From: Specific EtMIC3-binding peptides inhibit Eimeria tenella sporozoites entry into host cells

Figure 2

Detection of binding capability between selected phages amd EtMIC3-bc1 protein. EtMIC3-bc1-coated plate was incubated with thirty selected phages. The washed plate was then successively incubated with rabbit anti-M13 polyclonal antibody and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG. Then substrate solution (0.01% H2O2 and 1 mg/mL o-phenylenediamine) was added, and the reaction was stopped with 2 M sulfuric acid. The values of optical density (OD) at 490 nm was recorded. The blank control (BC), the negative control (NC) phages from G protein of porcine Vesicular Stomatitis Virus (VSV) (stored in our lab), and the positive control (PC) phages binding to EtAMA1 protein (stored in our lab) were used. Each sample was tested in triplicate. Values represent mean ± SD. Twenty-four selected phages clones showed higher binding capability than other clones (with the number of 1, 3, 13, 19, 23, 28), blank control and netative control phages (P < 0.01).    P < 0.01.

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