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Figure 2 | Veterinary Research

Figure 2

From: Protection studies of an excretory–secretory protein HcABHD against Haemonchus contortus infection

Figure 2

Parasitological determination of challenged goats in both trials. A Dynamic range of fecal egg counts (FEC) values of challenged goats in Trial 1. Goats in challenged vaccinated group (Group B) and challenged adjuvant group (Group C) started to excrete H. contortus eggs in fecal samples around Day 43, and FEC were then performed every 2 days until termination using the modified McMaster method. Eggs per gram (EPG) values were expressed as mean ± SD. Each data point represented the mean FEC value for each group (n = 5). B Determination of cumulative FEC for challenged goats in Trial 1. Cumulative FEC values were evaluated by calculating the area under the curve using the linear trapezoidal method. Cumulative FEC values (n = 5 for each group) were presented as minimum to maximum (all points). P-values were determined by non-parametric Mann–Whitney tests. C Worm burdens of each challenged group in Trial 1. Abomasum worm burdens were differentiated into male, female and total worms. The mean worm burdens (n = 5 for each group) were represented as minimum to maximum (all points), and two groups differed significantly when P < 0.05. ns not significant. D Dynamic range of FEC values of challenged goats in Trial 2. Goats in challenged immunized group (Group E) and challenged control group (Group F) began to excrete H. contortus eggs in feces around Day 29, and FEC were then performed every other day until the end of the trial. EPG values were represented as mean ± SD. Each data point represented the mean FEC value for each group (n = 5). E Determination of cumulative FEC for challenged goats in Trial 2. Cumulative FEC values were determined by calculating the area under the curve as well. Cumulative FEC values (n = 5 for each group) were presented as minimum to maximum (all points). P-values were determined by non-parametric Mann–Whitney tests. F Worm burdens of challenged goats in Trial 2. The mean worm burdens (n = 5 for each group) were denoted as minimum to maximum (all points), and two groups differed significantly when P < 0.05. ns not significant.

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